Benayahu Dafna
Mesenchymal Stem Cell Biology Lab
Research topics
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Mesemchymal cells (MSCs) lineage fate [osteogenic/adipogenic/myogenic] in aging/metabolic disease (osteoporosis/diabeties) and cancer
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MSCs molecular markers (CHD9-chromatin remodeling, SVEP1-cell adhesion)
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MSCs biomechanics/substrate rigidity (2D/3D) in differentiation fate
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Connective tissue protein component and structure
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Novel bio-composites scaffolds for tissue regeneration
Selected publications:
Benayahu D, Sharabi M, Pomeraniec L, Awad L, Haj-Ali R, Benayahu Y. Unique Collagen Fibers for Biomedical Applications. Mar Drugs. 2018 Mar 23;16(4). pi: E102.
Lustig M, Gefen A, Benayahu D. Adipogenesis and lipid production in adipocytes subjected to sustained tensile deformations and elevated glucose concentration: a living cell-scale model system of diabesity. Biomech Model Mechanobiol. 2018 Jan 15.
Yang X, Liu X, Li Y, Huang Q, He W, Zhang R, Feng Q, Benayahu D. The negative effect of silica nanoparticles on adipogenic differentiation of human mesenchymal stem cells. Mater Sci Eng C Mater Biol Appl. 2017 Dec 1;81:341-348
Shemesh S, Sidon E, Kaisler E, Sheinis D, Velkes S, Ohana N, Benayahu D. Diabetes mellitus is associated with increased elastin fiber loss in ligamentum flavum of patients with lumbar spinal canal stenosis: results of a pilot histological study. Eur Spine J. 2017 Oct 4.
Orgel JPRO, Sella I, Madhurapantula RS, Antipova O, Mandelberg Y, Kashman Y, Benayahu D, Benayahu Y. Molecular and ultrastructural studies of a fibrillar collagen from octocoral (Cnidaria). J Exp Biol. 2017 Sep 15;220(Pt 18):3327-3335.
Shoham N, Levy A, Shabshin N, Benayahu D, Gefen A. A multiscale modeling framework for studying the mechanobiology of sarcopenic obesity. Biomech Model Mechanobiol. 2017 Feb;16(1):275-295.
Yang X, Li Y, Liu X, Huang Q, He W, Zhang R, Feng Q, Benayahu D. The stimulatory effect of silica nanoparticles on osteogenic differentiation of human mesenchymal stem cells. Biomed Mater. 2016 Dec 2;12(1):015001.
Mandelberg Y, Benayahu D, Benayahu Y. Octocoral Sarcophyton auritum Verseveldt & Benayahu, 1978: Microanatomy and Presence of Collagen Fibers. Biol Bull. 2016 Feb;230(1):68-77.
Salomon-Kent R, Marom R, John S, Dundr M, Schiltz LR, Gutierrez J, Workman J, Benayahu D, Hager GL. New Face for Chromatin-Related Mesenchymal Modulator: n-CHD9 Localizes to Nucleoli and Interacts With Ribosomal Genes. J Cell Physiol. 2015 Sep;230(9):2270-80.
Shkolyar A, Gefen A, Benayahu D, Greenspan H. Automatic detection of cell divisions (mitosis) in live-imaging microscopy images using Convolutional Neural Networks. Conf Proc IEEE Eng Med Biol Soc. 2015 Aug;2015:743-6.
Katzengold R, Shoham N, Benayahu D, Gefen A. Simulating single cell experiments in mechanical testing of adipocytes. Biomech Model Mechanobiol. 2015 Jun;14(3):537-47
Shoham N, Mor-Yossef Moldovan L, Benayahu D, Gefen A. Multiscale modeling of tissue-engineered fat: is there a deformation-driven positive feedback loop in adipogenesis? Tissue Eng Part A. 2015 Apr;21(7-8):1354-63.
Sharabi M, Mandelberg Y, Benayahu D, Benayahu Y, Azem A, Haj-Ali R. 2014. A new class of bio-composite materials of unique collagen fibers. Journal of the Mechanical Behavior of Biomedical Materials 36:71-8.
Shoham N, Girshovitz P, Katzengold R, Shaked NT, Benayahu D, Gefen A. 2014. Adipocyte Stiffness Increases with Accumulation of Lipid Droplets. Biophysical J 106: 1-11.
Shefer G, Rauner G, Stuelsatz P, Benayahu D, Yablonka-Reuveni Z. 2013. Moderate-intensity treadmill running promotes expansion of the satellite cell pool in young and old mice. FEBS J. 280(17): 4063-4073.
Glait-Santar C, Pasmanik-Chor M, Benayahu D. 2012. Expression pattern of SVEP1 alternatively-spliced forms. Gene 15; 505(1):137-145.
Akavia UD, Socher R, Benayahu D. 2012. Tracking the Molecular Signature of Developing Skeletal Tissues. Front. Biosci 4:1941-50.
Glait-Santar C, Pasmanik-Chor M, Oron-Karni V, Benayahu D, 2012. Molecular profiling of functional interactions between pre-osteoblastic and breast carcinoma cells. Genes Cells 17(4):302-15.
Glait-Santar C, Benayahu D. 2012. Regulation of SVEP1 gene expression by 17beta-estradiol and TNFalpha in pre-osteoblastic and mammary adenocarcinoma cells. Journal of Steroid Biochemistry and Molecular Biology 130:36-44.
Glait-Santar C, Benayahu D. 2011. SVEP1 promoter regulation by methylation of CpG sites. Gene 490 (1-2): 6-14.
Shefer G, Rauner G, Yablonka-Reuveni Z, Benayahu D, 2010. Reduced satellite cell numbers and myogenic capacity in aging can be alleviated by endurance exercise. PloS One, 5 (10) e13307.
Shefer G and Benayahu D. 2010. SVEP1 is a Novel Marker of Activated Pre-determined Skeletal Muscle Satellite Cells. Stem Cell Rev. 6(1):42-9.
Ron A, Shur I, Singh RR, Daniel R, Fishelson N, Croitoriu N, Benayahu D, Shacham-Diamand Y. 2010. Dielectric screening of early differentiation patterns in mesenchymal stem cells induced by steroid hormones. Bioelectrochemistry. 78(2):161-72.
Ron A, Fishelson N, Shur I, Croitoriu N, Benayahu D, Shacham-Diamand Y. 2009. The Effect of Irregularity on the Dielectric Dispersion Characteristics of Spherical Cellular Suspension. Colloids and Surfaces B: Biointerfaces 74: 127-135.
Zusev M, Benayahu D. 2009. The crosstalk between MS-KIF18A kinesin and estrogen receptor alpha PloS One 4(7):e6407.
Eliaz N, Shmueli S, Shur I, Benayahu D, Aronov D, Rosenman G. 2009. The effect of surface treatment on the surface texture and contact angle of electrochemically deposited hydroxyapatite coating and on its interaction with bone-forming cells. Acta Biomater 5:3178-3191.
Benayahu D, Shefer, G, Shur I. 2009. Insights on chromatin remodellers in mesenchymal stem cells and differentiation. Front. Biosci 14:398-409.
Akavia UD, Benayahu D, 2008. Meta-Analysis and Profiling of Cardiac Expression Modules. Physiological Genomics 35:305-315.
Zusev M, Benayahu D. 2008. New insights on cellular distribution, microtubule interactions and post-translational modifications of MS-KIF18A. J Cell Physiol. 217:618-625.
Akavia UD, Veinblat O, Benayahu D, 2008. Comparing the transcriptional profile of mesenchymal cells to cardiac and skeletal muscle cells. J Cell Physiol 216:663-672.
Ron A, Singh RR, Fishelson N, Shur I, Socher R, Benayahu D, Shacham-Diamand Y. 2008. Cell-based screening for membranal and cytoplasmatic markers using Dielectric spectroscopy. Biophysl Chem 135: 59-68.
Socher R, Benayahu D. 2008. Comparative study using scanning electron techniques for imaging of micro architecture and antigen appearance. Journal of Microscopy 230:233-239.
Shefer G, Carmeli, E, Rauner, G, Yablonka-Reuveni Z, Benayahu, D. 2008. Exercise running and tetracycline as means to enhance Skeletal muscle stem cell performance after external fixation. J Cell Physiol 215:265-275.
Ron A, Singh RR, Fishelson N, Socher R, Benayahu D, Shacham-Diamand Y. 2008. Site localization of membrane-bound proteins on whole cell level using Atomic Force Microscopy. Biophys Chem 132:127-138.
Shur I, Zemer-Tov E, Socher R, Benayahu D. 2007. SVEP1 Expression is Regulated in Estrogen Dependent Manner. J Cell Physiol 210:732-739.
Luboshits G, Benayahu D. 2007. MS-KIF18A, a new Kinesin Associated with Estrogen Receptor. J Cell Biochem 100:693-702.
Shur I, Socher R, Hameiri M, Fried A, Benayahu D. 2006. Molecular and cellular characterization of SEL-OB/SVEP1 in osteogenic cells in vivo and in vitro. J Cell Physiol 206:420-427.
Shur I, Socher R, Benayahu D. 2006. In vivo association of CReMM/CHD9 with promoters in osteogenic cells. J Cell Physiol 207: 374-378.
Akavia UD, Shur I, Rechavi G, Benayahu D. 2006. Transcriptional profiling of mesenchymal stromal cells from young and old rats in response to Dexamethasone. BMC Genomics. 27:95.
Marom R, Shur I, Hager G, Benayahu D. 2006. Expression and regulation of CReMM, a Chromodomain Helicase-DNA-Binding (CHD), in Marrow Stoma derived Osteogenitors. J Cell Physiol. 207:628-635.
Shur I, Salomon R, Benayahu D. 2006. Dynamic Interactions of Chromatin-Related Mesenchymal Modulator, a Chromodomain Helicase-DNA-Binding Protein, with Promoters in Osteoprogenitors. Stem Cells. 24:1288-1293.
Marom R., Shur I., Solomon R., Benayahu D. 2005. Characterization of adhesion and differentiation markers by osteogenic marrow stromal cells. J Cell Physiol 202:41-48.
Shur I., Socher R., Benayahu D. 2005. Dexamethasone regulation of cFOS mRNA in osteoprogenitors. J Cell Physiol 202:240-245.
Grienberg I., Benayahu D. 2005. Osteo-Promoter Database (OPD) – Promoter Analysis in Skeletal Cells. BMC Genomics 6:46.
Luboshits G., Benayahu D. 2005. MS-KIF18A, new kinesin; structure and cellular expression. Gene 351:19-28.
Shur I., Benayahu D. 2005. Characterization and Functional Analysis of CReMM, a Novel Chromodomain Helicase-DNA-binding Protein. J Mol Biol. 352:646-655.
Benayahu D., Akavia U. D., Socher R., Shur I. 2005. Gene Expression in Skeletal Tissues: Application of Laser Capture microdissection. J of Microscopy 220:1-8.
Shur I., Zilberman M., Benayahu D. Einav S. 2005. Adhesion molecules expression by osteogenic cells cultured on various biodegradable scaffolds. Journal of Biomedical Materials Research 75: 870.
Shur I., Ben-Avraham D., Benayahu D. 2004. Alternatively spliced isoforms of novel Stromal RNA Regulating Factor. Gene 334:113-21.
Benayahu D., et al. 2001. Cellular changes associated with osteosarcoma cells. J Cell Biochem 84:108-114.
Benayahu D., et al. 2000. Hormonal changes affect the bone and marrow cells in a rat model. J Cell Biochem 79:407-415.
Benayahu D., 2000. The Hemopoietic Microenvironment, The osteogenic compartment of bone marrow: cell biology and clinical application. Hematology 4: 427-435.
Benayahu D., 1997. Estrogen effects on protein expressed by marrow stromal osteoblasts. Biochem. Biophys. Res. Commun. 233:30-35.
Benayahu D., et al. 1997. Osteocalcin (BGP) gene expression and protein production by marrow stromal adipocytes. Biochem. Biophys. Res. Commun. 231:442-6.
Benayahu D., et al. 1996. Expression of estrogen receptor and estrogen effect in MBA-15, marrow stromal osteoblasts. Cell. Biol. Inter. 20: 401-405.
Benayahu D., et al. 1995. Bone-Marrow interface: Preferential attachment of an osteoblastic marrow stromal cell line. J. Cell. Biochem. 59:151-160.
Benayahu D., et al. 1995. Monoclonal antibodies recognize antigen expressed by osteoblasts. J. Bone Miner. Res. 10: 1496-1503.
Benayahu D., et al. 1994. Bone formation by marrow osteogenic cells (MBA-15) is not accompanied by osteoclastogenesis and generation of hemopoietic supportive microenvironment. J. Bone Miner. Res. 9: 1107-1114.
Benayahu D., et al. 1994. Mineralization of marrow stromal osteoblasts MBA-15 on three-dimensional carriers. Calcif. Tissue Int. 55:120-127.
Benayahu D., et al. 1994. Bone-related matrix proteins expression in vitro and in vivo by marrow stromal cell line. Tissue and Cell 26: 661-666.
Benayahu D., et al. 1994. Myeloblastic cell line expresses osteoclastic properties following coculture with marrow stromal adipocytes. J. Cell. Biochem. 56: 374-384.
Benayahu D., et al. 1993. Marrow adipocytes regulate growth and differentiation of osteoblasts. Biochem. Biophys. Res. Commun 197:1245-1252.
Benayahu D., et al. 1992. Hemopoietic functions of marrow-derived osteogenic cells. Calcif. Tissue Int. 51:195-201.
Benayahu D., et al. 1991. Subpopulations of marrow stromal cells share a variety of osteoblastic markers. Calcif. Tissue Int. 49:202-207.
Benayahu D., et al. 1989. A bone marrow derived stromal cell line expressing osteoblastic phenotype in vitro and osteogenic capacity in vivo. J. Cell. Physiol. 140:1-7.